Fig. 5
From: Linked optical and gene expression profiling of single cells at high-throughput
Paired optical phenotype and gene expression analysis of enriched CD14+/CD16+ cells from PBMCs. a Use of dual antibody panel and PDM to significantly enrich for CD14+ and CD16+ cells from PBMCs encapsulated within droplets (top). Percentages represent quadrant proportions, with red indicating post-sort frequency. Annotation of sorted cells by cell type as identified through RNA-seq (bottom). b UMAP of bioinformatically filtered cells clustered by cell type (left). Cells are subsampled such that the ratio of CD14+ to CD16+ matches the observed pre-sorting ratio; those cells are clustered (right). c Overlays of CD14 and CD16 fluorescence for each cell on the UMAP. d Scatter plots of gene expression and fluorescence data for 3 selected genes, with moving average of expression plotted above (bin size = 30)