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Fig. 2 | Genome Biology

Fig. 2

From: Linked optical and gene expression profiling of single cells at high-throughput

Fig. 2

Printed Droplet Microfluidics (PDM) operation for deterministic loading of nanowell array with beads and cells. a An inset of the microfluidic device aligned over the nanowell array, with images (top to bottom) of regions of drop fluorescence recording, sorting of drops of interest, and dispensing of drops to nanowells. b Monodisperse droplet emulsions containing fluorescently labeled cells (top) or beads (bottom) are input into PDM. Drops of interest (insets) are enriched for by gating on fluorescence plots (right) generated during device operation. c Deterministic merging of cells and beads through first adding beads to nanowells, followed by merging of a cell-containing drop in lysis buffer

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