Fig. 1
From: Linked optical and gene expression profiling of single cells at high-throughput
A high-throughput platform for linked optical phenotype and gene expression of single cells. a Monodisperse droplet emulsions containing encapsulated poly-T mRNA capture beads and cells are input into a microfluidic device. Fluorescence signal from droplets is interrogated and used to selectively dispense a cell and bead to indexed locations on a nanowell array. b Each bead binds mRNA from cell lysate as well as a unique combination of poly-A barcode oligos denoted by nanowell coordinate. c UMI counts on each bead are collected through sequencing into an expression matrix for each cell. Nanowell coordinate is assigned based on the abundance of barcode oligos and paired with fluorescence data obtained during cell sorting, which enables downstream linked analyses such as dimensionality reduction visualizations of gene expression paired with optical phenotype