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Fig. 2 | Genome Biology

Fig. 2

From: Histone H3K27 acetylation is dispensable for enhancer activity in mouse embryonic stem cells

Fig. 2

Transcriptome and enhancer identity are maintained in H3.3K27R mutant ESCs. a Dot plot depicting the transcriptional changes in all genes in H3.3K27R mutant ESCs compared to WT ESCs. Genes upregulated twofold with an FDR < 0.05 are shown in red. Genes downregulated twofold with an FDR < 0.05 are shown in green. b Dot plot depicting the transcriptional changes in genes associated with H3K27ac-occupied enhancers in H3.3K27R mutant ESCs compared to WT ESCs. c Dot plot revealing the relationship between transcriptional changes in genes associated with H3K27ac-occupied enhancers and H3K27ac ChIP-seq signal changes in H3.3K27R mutant ESCs compared to WT ESCs. d Dot plot depicting the transcriptional changes in genes associated with H3K27ac at TSSs in H3.3K27R mutant ESCs compared to WT ESCs. e Dot plot revealing the relationship between transcriptional changes in genes associated with H3K27ac at TSSs and H3K27ac ChIP-seq signal changes in H3.3K27R mutant ESCs compared to WT ESCs. f, g ATAC-seq signals in WT and H3.3K27R mutant mouse ESC lines at enhancers (f) and H3K27ac-occupied TSSs (g). Upper, averaged profiles of ATAC-seq signals at enhancers and H3K27ac-occupied TSSs, respectively. Lower, heatmap plots of ATAC-seq signals at enhancers and H3K27ac-occupied TSSs, respectively. All biological duplicates are shown. h, i RNA Pol II signals in WT and H3.3K27R mutant ESC lines around the TSSs of genes associated with distal active enhancers (h) and around TSSs overlapped with H3K27ac peaks (i). Upper, averaged profiles of the RNA Pol II signals in each group. Lower, heatmap plots of the RNA Pol II signals in each group

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