Fig. 6

Analyses of the effects of co-transfected G8P and Acrs on the genome-editing activity of SpCas9. a AcrII4A abolishes the on- and off-activities of SpCas9, as determined by T7E1 assay. The results are shown as mean ± SD (n = 3). b, c Density plot showing the distribution of mutation rates along the gene-edited genomic sites of AAVS1 in Hela (b) and K562 (c) cells. The mean values of three biological replicates are displayed. Significant difference between test groups and mock is determined by one-way ANOVA with Dunnett’s multiple comparisons test. Low and high indicate increased and decreased mutation rates, respectively. The adjusted P values are indicated