Fig. 4
From: Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides
α-helical structure is critical for the Cas9-inhibiting activity of G8PPD. a Alanine mutations at positions 6–11 abolish the inhibitory activity of G8PPD. The results are shown as mean ± SD (n = 3). The arrow denotes cleavage products. b Structure of G8PPD peptide (PDB entry 2MJZ), displayed by PyMOL. Residues 6–11 are shown as stick. c CD spectra of f1 G8PPD WT and mutant 2