Fig. 3
From: Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides
Identification of M13 G8PPD binding site in the PI domain of SpCas9. a Maximum XlinkX scores of peptide hits in high-resolution MS analyses. b Secondary MS showing crosslinked peptides KSVKEL-AEGDDPAKAAF. c Secondary MS showing crosslinked peptides EKNPIDFLEAKGY-AEGDDPAKAAF. d Location of G8PPD binding sites in SpCas9. The structure of SpCas9 in complex with AcrIIA4 (5VW1) is displayed by PyMOL. AcrIIA4 is shown in blue. The candidate G8PPD binding sites on SpCas9 are shown in purple and red respectively. e In vitro DNA cleavage by WT, K1176 mutant and K1158 mutant SpCas9. Arrow indicates cleavage product. The results are shown as mean ± SD (n = 3)