Fig. 1
From: Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides
Inhibition of the in vitro activity of SpCas9 by intact M13 phage and phage-derived G8PPD peptides. a Dose-dependent inhibition of SpCas9 by intact M13 phage. b Intact M13 phage does not inhibit the in vitro activity of assembled Cas9-sgRNA RNP. c Structural organization of M13 phage major coat protein G8P. d Dose-dependent inhibition of SpCas9 by G8PPD. e G8PPD does not inhibit the in vitro activity of assembled Cas9-sgRNA RNP. The above reactions are performed in the absence or presence of 50 nM SpCas9 proteins. The results are shown as mean ± SD (n = 3). Arrows indicate cleavage products