Fig. 6
From: Enteric infection induces Lark-mediated intron retention at the 5′ end of Drosophila genes
Lark dosage perturbation leads to global changes in gene expression as well as enhanced survival to infection. a Left: general schematic of the crosses to generate enterocyte (EC)-specific expression of transgenes in adult female flies. Myo1Ats virgins were crossed to either UAS-lark RNAi, UAS-lark-3HA, or w1118 males, and their F1 progeny were maintained at 18 °C. After eclosion, adults were kept at 29 °C for 7 days, then infected with P.e. Middle: survival of lark overexpression and knockdown flies driven by the Myo1Ats Gal4 driver. Right: relative ratio of lark in dissected guts of those flies 4 h after infection with P.e. All experiments were performed with three biological replicates and n > 30 flies or guts. b Gene set enrichment analysis of the lark perturbation effect and infection effect as obtained by gene-level differential expression analysis. Each point is a gene set from the biological process gene ontology whose normalized enrichment score (NES) is plotted in two analyses. Overexpression and knockdown lead to similar changes in gene expression and common pathway enrichments