Fig. 4

Enteric infection with different pathogens leads to widespread, directed changes in intron retention. a Diagram depicting how intron retention changes are calculated. For each sample, delta PSI values for different splicing events [23] were calculated by subtracting the PSI value of the uninfected control sample from that of the infected one. b Histogram of average delta PSI values of intron retention (RI) events whose PSI values are significantly different after infection in at least 4 DGRP lines. c, d Histogram of delta PSI values of intron retention events whose PSI values are significantly different (Bayes factor > 10, delta PSI > 0.2) from the control (sucrose fed) state 4 h after infection with c Pe and d Ecc15 in w¹¹¹⁸ flies. e Venn diagram of the overlap between events that are significant in 1 DGRP line, at least 4 DGRP lines, w¹¹¹⁸ strain infected with Pe, and w¹¹¹⁸ strain infected with Ecc15