Fig. 3
From: Enteric infection induces Lark-mediated intron retention at the 5′ end of Drosophila genes
Post-infection transcripts tend to be longer, mainly due to the generation of longer 5′ UTRs. a The line-specific effective length of each gene’s transcript, CDS, 5′UTR, and 3′UTR length was obtained by calculating the weighted sum of each gene’s isoform features by its isoform ratios. The difference in effective length between the P.e. infected state and the uninfected (control) state was then calculated for each line. b The percentage of features that increased, decreased, or did not change in average length (across samples) after infection. Error bars are the standard deviation. A null distribution was generated by performing 100 permutations by randomly shuffling the samples. The gray bars indicate the average obtained by permutations. Repeated G-tests were used to compare the feature length change in each line to the null distribution. The boxplots show the –log10(p values) of the tests, with the dotted red line representing a Bonferroni-corrected p value threshold. c Similar to previous panel, but this time the effective length of each transcript without either the predicted polypeptide, 3′UTR, or 5′UTR was calculated