Fig. 2
The gene nutcracker is involved in the gut response. a Survival of lines harboring a null mutant (yellow) allele ntcms771 with (top panel) and without (bottom panel) TM6B balancer upon P.e. infection compared to control (black). Log ranked test p < 0.0001 and p = 0.081 for lines with and without balancer chromosome, respectively. Shaded area represents the 95% confidence interval. b Gene expression of ntc (purple) and DiptA (green) as measured by qPCR, normalized to RpL32 in control (left) and infected (right) conditions in ntcms771 mutant (yellow) and control (black) lines with TM6B balancer. c Gene expression of ntc and DiptA as measured by qPCR, normalized to RpL32 in infected (right) and control (left) conditions in ntcms771 mutant (yellow) and control (black) lines without TM6B balancer. Data presented in a–c are based on at least three biological replicates. d Left panel: Expression level (in log2(cpm)) of the ntc gene by resistance class in control (gray) and infected (orange) conditions. Right panel: Fold change of ntc expression by resistance class after infection. Green and red points represent resistant and susceptible DGRP lines, respectively