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Fig. 1 | Genome Biology

Fig. 1

From: cis-regulatory variation modulates susceptibility to enteric infection in the Drosophila genetic reference panel

Fig. 1

Expression profiling of phenotypic extremes does not reveal consistently differentially expressed genes between classes. a Study design: 30 adult female flies from two phenotypic extremes (18 resistant and 20 susceptible) of the DGRP were infected orally with P.e. or fed sucrose. Whole guts of ~ 30 flies were dissected per condition and line, then RNA sequencing was performed. Sequencing reads were mapped to individualized genomes, and the number of reads was counted per gene. b Principal component analysis plots of all the samples (left), the control condition (middle), and the infected condition alone (right). The R package FactomineR was used to obtain the coordinates of each sample in the first two components, as well as the variance explained by each component (in parentheses). c Infection leads to the differential expression of around 2400 genes (BH-corrected p value < 0.05, fold change > 2). d When lines of the two resistance classes are compared within condition, no genes are significantly differentially expressed in the control condition, and only one gene, ntc, in the infected condition

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