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Fig. 4 | Genome Biology

Fig. 4

From: PASTMUS: mapping functional elements at single amino acid resolution in human cells

Fig. 4

Identification of PSMB5 amino acids critical for Bortezomib-mediated killing through PASTMUS. a Identification of PSMB5-targeting sgRNAs conferring cell resistance to Bortezomib. Distribution of sgRNAs mapped to the corresponding amino acid in PSMB5 is indicated on top. b a.a. deletion fold change corresponding to each a.a. c a.a. combo mutation fold change corresponding to each a.a. d a.a. substitution frequency corresponding to each a.a. e Essential score of each a.a. of PSMB5. Cutoff of essential score is plotted as a dashed line, with critical amino acids above the cutoff shown in black and known critical amino acids labeled in red. f MTT viability assay for indicated substitutions of PSMB5 on the susceptibility of cells to Bortezomib. g Effects of indicated substitutions in PSMB5 on the susceptibility of cells to Bortezomib. Data are presented as the mean ± SD, n = 6. h Crystal structure of PSMB5. Five positive amino acids and the novel critical site, V90, are labeled in green and purple, respectively (PDB code: 5LF3)

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