Fig. 4

CasRx mediates efficient interference against TuMV-GFP virus by preventing its systemic spread. a Cas13-mediated interference against the GFP-expressing TuMV virus in plants. N. benthamiana plants expressing LwaCas13a, PspCas13b, and CasRx proteins were co-infiltrated with TRV (expressing crRNAs targeting the TuMV-GFP virus and NS crRNA) and TuMV-GFP. At 7 dpi, plants were imaged under UV light for GFP signal monitoring. b RT-qPCR analysis of TuMV-GFP knockdown with different Cas13 variants using the different position-matched crRNAs. For each Cas13 variant, knockdown efficiency of each targeting crRNA is shown relative to the non-targeting (NS) crRNA. Values shown as mean ± SEM (n = 3)