Fig. 6
Subject-derived E. faecium strains lead to different pathology and cytokine expression profiles in the colon. Fecal suspensions from selected subjects (HD55 and IBD36) and E. faecium strain IB18a, IB51a, or HD26a suspensions were transplanted or inoculated into microbiota-depleted Il10−/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Mean pathology scores of mice from each treatment group. d mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. e, f Suspension of HD55 or IBD51 feces or strain IB51a was gavaged into germ-free Il10−/− mice. e Mean pathology scores of mice from each treatment group. f mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a–f are the mean ± SE. Statistical differences between a value and the HD55 control were analyzed using the Kruskal-Wallis test followed by Dunn’s test. *P < 0.05; **P < 0.01; ***P < 0.001