Fig. 2

The fecal microbiota of IBD patients cause colitis in Il10^−/− mice. Fecal suspensions from HD, UC, or CD subjects were transplanted into microbiota-depleted Il10^−/− mice. The control group was treated with antibiotics (vancomycin/doripenem; VCM/DRPM) in the absence of transplantation. All mice were euthanized 28 days post-transplantation. a Changes in body weight (%) of each treatment group throughout the course of the experiment and b on day 28. c Representative histological sections of the murine colon at the time of euthanasia. Bars, 100 μm. d Mean pathology scores of mice corresponding to individual fecal donors or e complete treatment groups. †, average pathology score of 0. The identification codes of individual subjects are shown on the x-axis in d. f Linear regression line determined from plots showing average changes in body weight (%) on day 28 and pathology scores of mice in all groups. The regression coefficient (R) and P value are shown. Values shown in a, b, d, and e are the mean ± SE. The numbers above the error bars in d indicate the number of mice in each category. Statistical differences between two values were analyzed using a Mann-Whitney U test. *P < 0.05; **P < 0.01; ***P < 0.001