Fig. 6
From: Chromatin interactome mapping at 139 independent breast cancer risk signals
Altered TBX3 levels affect breast cell growth and tumor formation. a Representative images of colonies grown in soft agar for HMLE-control (GFP CON) and HMLE-TBX3-overexpressing cells (TBX3 ORF). The graph depicts the total number of HMLE colonies formed. Error bars represent SEM (n = 2). b Representative images of colonies grown in soft agar for HMLE-control (PgCON) and HMLE-CRISPR/Cas9 TBX3-edited cells (SgTBX3-C1/C2). The graph depicts the total number of HMLE colonies formed. Error bars represent SEM (n = 2). c Representative images of colonies grown in soft agar for MCF7-control (GFP CON) and MCF7-TBX3-overexpressing cells (TBX3 ORF). The graph depicts the total number of MCF7 colonies formed. Error bars represent SEM (n = 4). d Representative images of colonies grown in soft agar for MCF7-control (PgCON) and MCF7-dCas9-KRAB TBX3 repressed cells (SgTBX3-P1/P2). The graph depicts the total number of MCF7 colonies formed. Error bars represent SEM (n = 4). a–d p values were determined by two-way ANOVA followed by Dunnett’s multiple-comparison test (**p < 0.01, ***p < 0.001, ****p < 0.0001). e MCF7-control (PgCON) or MCF7-dCas9-KRAB TBX3-repressed cells (SgTBX3-P1/P2) were injected into the mammary fat pads of nude mice. Tumor growth curves for each group are shown. Values are shown as average tumor volumes at each time point. Error bars represent SEM (n = 8–9 mice per group). f Tumors of individual mice were dissected at day 44 post-injection. The five largest tumors of each group are shown. The scale bar represents 1 cm. g Plot of the individual weights of tumors with mean and SEM shown by cross-bar and errors. e, g Mann-Whitney U test was used to compare the differences between the groups (*p < 0.05, ****p < 0.0001)