Fig. 2

Relationship between pairwise \(s_{2}^{s}\) estimated by primate genomes and NGS samples using K=14 and M=12 of different numbers of reads without bias adjustment. X-axis is the pairwise \(s_{2}^{s}\) estimated by genomes, and Y-axis is the pairwise \(s_{2}^{s}\) estimated based on NGS samples. a–h Relationship between \(s_{2}^{s}\) estimated by primate genomes and \(s_{2}^{s}\) estimated based on NGS samples of only 1 M, 3 M, 5 M, 7 M, 9 M, 11 M, 13 M, or 15 M reads, respectively. i Pairwise \(s_{2}^{s}\) estimated based on mixed NGS samples. NGS samples of different numbers of reads are colored accordingly. “Mix” means two NGS samples have different numbers of reads (e.g., between 1 and 5 M or between 7 and 11 M) and is colored in gray. The root mean squared error (RMSE) and Spearman correlation coefficients (SPC) between pairwise \(s_{2}^{s}\) estimated based on NGS samples and genomes are shown on each subplot