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Fig. 4 | Genome Biology

Fig. 4

From: Characterizing the interplay between gene nucleotide composition bias and splicing

Fig. 4

Splicing regulation by splicing factor–mimicking oligonucleotides. a MCF-7 and HeLa cell lines were transfected with siRNAs (as indicated), and the splicing pattern of the indicated exons was analyzed by RT-PCR. siU2 corresponded to a mix of siSF1 and siU2AF2. Each siRNA had a specific effect on the selected exons. b MCF-7 and HeLa cell lines were transfected with a control siRNA, siPTBP1 (upper panel), or sihnRNPK (lower panel) and a control AON (−) or AON_TDP52L2 (+) targeting supernumerary predicted BPs upstream of TPD52L2 exon 4. Relative PSI as measured by qPCR represents the inclusion/exclusion ratio normalized by the inclusion/exclusion ratio obtained in control cells (i.e., cells transfected with siCTRL and a control AON). Box plots represent the mean value (+ S.E.M.) of three independent experiments. “*” corresponds to P value< 0.05 and “**”corresponds to P value< 0.005 (Student’s t test). c As in b using sihnRNPK and AON_SPG11 targeting intronic sequences upstream SPG11 exon 28. *P value < 0.05 and **P value < 0.005 (Student’s t test). d As in b using siTRA2 and AON_STAU2 targeting intronic sequences upstream STAU2 exon 3

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