Fig. 1

Splicing factor–dependent GC-rich and AT-rich exons. a Heatmaps representing the relative median frequency of GC and AT nucleotides in sets of splicing factor–activated exons, as compared to the median values computed from control exons. “*” corresponds to Student’s test FDR < 0.05. b Correlation between the GC or GA content (upper or lower panel, respectively) of splicing factor–activated exons and the GC or GA content, respectively, of their upstream intron; r = Pearson correlation coefficient. c Correlation between the relative GC content of splicing factor–activated exons and the size of their flanking introns (upper panel) or the exon size (lower panel); r = Pearson correlation coefficient. d The x-axis represents the relative median size of the smallest intron flanking splicing factor–activated exons, as compared to the median size of human introns. The y-axis represents the relative median GC content of splicing factor–activated exons, as compared to the median GC frequency of control exons; r = Pearson correlation coefficient. e Violin plots representing the GC content (%) of GC exons and AT exons (left panel), and the logarithmic nucleotide size of the smallest intron flanking GC exons and AT exons (right panel). The red lines indicate the median values computed for control exons. “***” corresponds to Wilcoxon’s test P < 10^− 16 when comparing GC exons to AT exons