Skip to main content
Fig. 2 | Genome Biology

Fig. 2

From: MIA-Sig: multiplex chromatin interaction analysis by signal processing and statistical algorithms

Fig. 2

Enrichment test on Drosophila S2 cells RNAPII ChIA-Drop data.a MIA-Sig performs an enrichment test on RNAPII-enriched ChIA-Drop data by retaining complexes with fragments in strong binding regions, which also correspond to RNAPII ChIA-PET peaks. b The significant complexes are pronounced in regions with a high level of transcription, abundant loops, and active histone mark; insignificant complexes tend to be in inactive regions. c Log of H3K27ac signal for fragments in significant and insignificant complexes (one-sided Mann-Whitney U test, p value <2.2 × 10−16). d Log of H3K27me3 signal for fragments in significant and insignificant complexes (one-sided Mann-Whitney U test,p value <2.2 × 10−16). e Fragment coverage profile of significant complexes is similar to that of RNAPII ChIA-PET, with 45 promoter-centric multiplex interactions (green, non-promoters; light green, promoters). By contrast, insignificant complexes do not show any strong binding peaks in coverage, and 91 multiplex interactions are non-specific (turquoise, non-promoters; light turquoise, promoters).f Genome-wide, significant complexes have a higher proportion of active promoter fragments than insignificant complexes do (two-sided K-S test statistic =0.39, p value <2.2 × 10−16). g The insignificant RNAPII ChIA-Drop complexes from the enrichment test are comparable to the significant ChIA-Drop complexes from the distance test. TADs (black lines) are called by MIA-Sig on the latter complexes

Back to article page