Fig. 6

Molecular functional study of PGLS. a Soma size of GFAP-positive astrocytes was substantially larger in AAV-PGLS mice than in control (Ctrl) mice in most brain regions (unpaired test or unpaired t test with Welch’s correction: hippocampus p = 0.0158, temporal cortex p < 0.0001, prefrontal cortex p = 0.7358, stratum p = 0.0008, and total p = 0.001; scale bars: large = 100 μm and small = 10 μm). b Example photomicrograph of fluorescent IHC and cropped cell with skeletonized image. AAV-PGLS group (n = 10 cells) had significantly shorter branch length and more slab voxels than AAV-control group (n = 10 cells) (branch length: unpaired t test with Welch’s correction t = 2.709, p = 0.019; slab voxels: unpaired t test with Welch’s correction t = 5.17, p = 0.0004). c Functional enrichment of differentially expressed genes after overexpression of PGLS. d Representative immunoblots of PSD95 and caspase-3 in brains from AAV-PGLS and Ctrl mice at 12 months of age (p = 0.0094 for PSD95; p = 0.0383 for caspase-3; unpaired t test with Welch’s correction)