Skip to main content

Table 1 Overview of accuracy and performance of scSplit on simulated mixed samples, with one CPU and 30GB RAM

From: Genotype-free demultiplexing of pooled single-cell RNA-seq

Simulationsim2sim3sim4sim8sim12sim16sim24sim32
Mixed samples234812162432
Number of cells12 38312 38312 38312 38312 38312 38312 38312 383
Reads per cell4 9734 9734 9734 9734 9734 9734 9734 973
Informative SNVs34 11634 11634 11634 11634 11634 11634 11634 116
Assigning cells41 min41 min46 min47 min1h54m2h112h332h55
Singlet TPR0.970.970.970.970.970.970.960.96
Singlet FDR09E −59E −59E −5005E −38E −3
Doublet TPR0.9970.9970.9970.9970.9970.9970.9950.997
Doublet FDR00000000
Cohen’s Kappa1.01.01.01.01.01.00.970.98
  1. We used PBMC donor B [2] and genotype data from demuxlet [6] as simulation templates