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Fig. 4 | Genome Biology

Fig. 4

From: Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses

Fig. 4

Disentangling the effects of digestion time and digestion method on transcriptomic response. a Mean normalized expression of genes in the core gene set as a function of digestion time colored by digestion temperature. Digestion by collagenase causes upregulation of the gene set at all time points, with a subset showing further upregulation as digestion time increases. B Log fold changes of a 2-h vs. 30-min digestion for collagenase only as a function of log counts-per-million. c Log fold changes of a collagenase vs. cold protease digestion at 30-min digestion time as a function of log counts-per-million. d Log fold changes of a collagenase vs. cold protease digestion at 2-h digestion time as a function of log counts-per-million. e Log fold changes of a 2-h vs. 30-min digestion (collagenase only) compared to a collagenase vs. cold protease digestion at 2 h demonstrate a large overlap between genes affected (ρ = 0.8)

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