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Fig. 4 | Genome Biology

Fig. 4

From: The first enhancer in an enhancer chain safeguards subsequent enhancer-promoter contacts from a distance

Fig. 4

The biological functions of ECs in gene regulation. a The level of gene expression associated with multiple complete ECs (labeled “multi ECs”), one EC only, one enhancer in a gene locus only (one enh), and an EC connecting to the target promoter through a non-active enhancer (non-enh) DNA fragment, respectively. b The level of gene expression associated with different numbers of ECs. c The averaged gene expression for a group of genes connected by the same EC. d The enriched TFBSs in the EC enhancers (positive set) compared to the non-chain enhancers (control set). e The enrichment of histone modifications in the EC enhancers (positive) compared to the non-chain enhancers (control set). Red and blue represent the histone marks that are enriched in EC and non-chain enhancers, respectively. f The fraction of enhancers along the chain that overlaps the initiator enhancers. The comparison of the genomic features between ECs with E1 associated with only one promoter (1P-E1) and with multiple promoters (MP-E1) includes the distance to the target promoter (g), the number of connected promoters/enhancers (h), density of tissue-specific eQTLs (i), and associated gene expression levels (j). *p < 0.05, **p < 0.001, ***p < 1 × 10−10; p values are calculated using the Wilcoxon rank-sum test and the binomial test

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