Fig. 4

sgRNA pair optimization for coverage of groups. a Variant pairs in NEFL and the flanking 5 kb that are shared by at least 10% of the 1KGP cohort. These are pairs of variants, not pairs of sgRNAs, so reflect potential dual-guide editing sites prior to designing or filtering sgRNAs. Ten percent was chosen for visualization purposes. b Five variant pairs identified by AlleleAnalyzer to achieve greatest possible coverage of the 1KGP cohort. c Coverage of the 1KGP cohort with the AlleleAnalyzer set of five pairs at various minimum predicted specificity score thresholds. d Coverage of each super population in the 1KGP cohort with the AlleleAnalyzer set of five pairs at various minimum predicted specificity score thresholds. e Top 5 shared variant pairs in the 1KGP cohort. f Coverage of the 1KGP cohort with the “top 5” set of pairs at various minimum predicted specificity score thresholds. g Coverage of each super population in the 1KGP cohort with the “top 5” set of pairs at various minimum predicted specificity score thresholds