Fig. 4

Non-neutral evolution. a Allele fraction distribution of chromosome 10 in tumor crcA5EK. SNVs in black are subclonal SNVs between an allele fraction range of 0.06 to 0.21. Allele fraction range of 0.12–0.24 used in Williams et al. is shown with a green bracket. b Violin plot of correlations between inverse allele fraction and cumulative number of SNVs. The red vertical line represents the 0.98 cutoff used in a previous study [14]. The numbers of tumors fitting the neutral evolution model and the total numbers of tumors tested are listed on the right for three subtypes. c An example of subclonal expansion. Two peaks of subclonal SNVs are present in the SNV allele fraction distribution plot. A subclonal expansion corresponding to peak 1 could be quantified since it is not too close to the SNV detection limit