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Table 2 Generalized regression analysis to identify factors predicting the success of the 2-sgRNA 2-ssODN method

From: Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

  Estimate Standard error t value p value
Intercept − 0.14 0.15 0.91 0.37
Efficiency of 5′ sgRNA 0.19 0.23 0.84 0.4
Efficiency of 3′ sgRNA − 0.31 0.22 − 1.36 0.18
5′ LoxP insertion 0.01 0.92 0.017 0.98
3′ LoxP insertion 0.01 0.38 0.27 0.79
5′ LoxP × 3′ LoxP insertion 4.05 2.32 1.74 0.09
Cas9 mRNA concentration 0.004 0.002 1.92 0.06*
Cas9 protein concentration 0.0003 0.003 0.1 0.91
SgRNA concentration − 0.002 0.002 − 1.12 0.27
ssODN concentration 0.002 0.001 0.13 21
Distance − 0.00001 < 0.0001 − 1.28 0.21
  1. ***p < 0.01, *p < 0.05