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Table 2 Generalized regression analysis to identify factors predicting the success of the 2-sgRNA 2-ssODN method

From: Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

 

Estimate

Standard error

t value

p value

Intercept

− 0.14

0.15

0.91

0.37

Efficiency of 5′ sgRNA

0.19

0.23

0.84

0.4

Efficiency of 3′ sgRNA

− 0.31

0.22

− 1.36

0.18

5′ LoxP insertion

0.01

0.92

0.017

0.98

3′ LoxP insertion

0.01

0.38

0.27

0.79

5′ LoxP × 3′ LoxP insertion

4.05

2.32

1.74

0.09

Cas9 mRNA concentration

0.004

0.002

1.92

0.06*

Cas9 protein concentration

0.0003

0.003

0.1

0.91

SgRNA concentration

− 0.002

0.002

− 1.12

0.27

ssODN concentration

0.002

0.001

0.13

21

Distance

− 0.00001

< 0.0001

− 1.28

0.21

  1. ***p < 0.01, *p < 0.05
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Genome Biology

ISSN: 1474-760X

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