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Table 1 Summary of the edited blastocysts for Mecp2 gene from three different centers

From: Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

  Zygotes injected Blastocysts genotyped Correctly targeted Incorrectly targeted at the 5′ site (%) Incorrectly targeted at the 3′ site (%)
Australian National University (ANU), Australia 106 51 0 11 indels and 6 LoxP correctly inserted (33%) 6 indels and 1 LoxP correctly inserted (13%)
University of Nebraska Medical Center (UNMC), USA 80 70 0 14 indels and 1 LoxP correctly inserted (21%) 21 indels (30%)
Czech Centre for Phenogenomics, Czech republic (BIOCEV/IMG) 40 28 0 8 indels and 1 LoxP correctly inserted (32%) 5 indels (18%)