Table 1 Summary of the edited blastocysts for Mecp2 gene from three different centers
Zygotes injected | Blastocysts genotyped | Correctly targeted | Incorrectly targeted at the 5′ site (%) | Incorrectly targeted at the 3′ site (%) | |
|---|---|---|---|---|---|
Australian National University (ANU), Australia | 106 | 51 | 0 | 11 indels and 6 LoxP correctly inserted (33%) | 6 indels and 1 LoxP correctly inserted (13%) |
University of Nebraska Medical Center (UNMC), USA | 80 | 70 | 0 | 14 indels and 1 LoxP correctly inserted (21%) | 21 indels (30%) |
Czech Centre for Phenogenomics, Czech republic (BIOCEV/IMG) | 40 | 28 | 0 | 8 indels and 1 LoxP correctly inserted (32%) | 5 indels (18%) |