Fig. 2From: Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluationQuantitative assessment of the success of the two-donor floxing method. a Method of zygote injections (pronuclear, cytoplasmic, or both) for delivery of the CRISPR reagents used by reporting centers. Numbers indicate the percentage of the total zygotes microinjected or electroporated. b Form of the CRISPR reagents (mRNA, protein, or plasmid) delivered to the zygotes. Numbers indicate percentages. c Number of successfully edited alleles and correct LoxP insertions out of the total number of live-born pups from microinjected and transferred zygotes. Numbers indicate absolute numbers. d Types of editing observed among the live-born pups genotyped from a subsample from 25 loci. Numbers indicate absolute valuesBack to article page