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Fig. 1 | Genome Biology

Fig. 1

From: Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

Fig. 1

Schematic of two-donor floxing method of creating cKO alleles. a Wild-type locus showing exons 3, 4, and 5 of a hypothetical gene where exon 4 is chosen as a target exon for inserting LoxP sites. Guides 1 and 2 target introns 3 and 4, respectively. b CRISPR components for the two-donor floxing method Cas9 source. c Delivery method of CRISPR components into zygotes (microinjection or electroporation). d The cKO allele showing target exon (#4) with flanking LoxP sites

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Genome Biology

ISSN: 1474-760X

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