Fig. 6
Expression analyses of tomato ALKBH genes reveal the involvement of SlALKBH2 in fruit ripening. a Phylogenetic analysis of tomato m6A RNA demethylases (ALKBHs). Phylogenetic tree of the tomato ALKBHs is shown. Included in the tree are all ALKBHs from Arabidopsis and two well-characterized ALKBHs, human ALKBH5 and mouse ALKBH5. The phylogenetic tree was produced using MEGA (version 5.2). Bootstrap values from 1000 replications for each branch are shown. Tomato proteins are indicated in red. Species names are abbreviated as follows: At, Arabidopsis thaliana; Sl, Solanum lycopersicum; Hs, Homo sapiens; Mm, Mus musculus. The accession numbers are indicated in parentheses. b Heat map analysis showing the gene expression of SlALKBH1-8 in wild-type (WT) fruit at 39 DPA and 42 DPA and Cnr fruit at 42 DPA by RNA-seq. Data are presented as the mean of three independent biological replicates. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t test). NS, no significance; DPA, days post-anthesis; FPKM, fragments per kilobase of exon per million mapped fragments. c Gene expression of SlALKBH2 in WT fruit at different ripening stages and Cnr fruit at 42 DPA as determined by quantitative RT-PCR analysis. The ACTIN gene was used as an internal control. Error bars represent the standard deviation of three independent experiments. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t test)