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Fig. 5 | Genome Biology

Fig. 5

From: RNA methylomes reveal the m6A-mediated regulation of DNA demethylase gene SlDML2 in tomato fruit ripening

Fig. 5

Changes in m6A levels in transcripts of specific ripening-related genes in the Cnr mutant. a Integrated Genome Browser (IGB) tracks displaying m6A-seq read distributions in DEMETER-like DNA demethylase 2 (SlDML2), fruitfull 2 (FUL2), and never-ripe (NR) transcripts. The black dot line rectangles indicate the position of m6A peaks with significantly increased m6A enrichment (fold changes ≥ 1.5 and P value < 0.05) in Cnr fruit compared to wild-type (WT) fruit. b Validations of the m6A enrichment by m6A-immunoprecipitation (IP)-qPCR. c Gene expression level of SlDML2, FUL2, and NR revealed by RNA-seq. b, c Error bars represent the standard deviation of three independent experiments. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t test)

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