Fig. 1
Dynamics of DNA methylation (5mC) and mRNA m6A methylation in tomato fruit ripening. a Images of wild-type (WT) fruit at different ripening stages and Cnr fruit at 42 DPA. DPA, days post-anthesis; scale bar = 1 cm. b Relative 5mC levels of WT and Cnr fruit shown in a. For 5mC assay, 100 ng of genomic DNA was detected in each sample by MethylFlash™ methylated DNA quantification kit. 5mC level in each sample was normalized to that of the positive control according to the manufacturer’s instructions. The plus sign represents the average in each box. c LC-MS/MS assay showing the amount of mRNA m6A in WT and Cnr fruit shown in a. Data are presented as mean ± standard deviation (n = 3). Asterisks indicate significant differences (*P < 0.05, **P < 0.01; Student’s t test). NS, no significance