Fig. 10

Comparative analysis of Glossina male accessory gland (MAG) protein family memberships. Graphical representation of the evolutionary rate and gene number variability in male accessory protein genes across Glossina species. a Average ratio of synonymous to non-synonymous changes in male reproductive-associated genes relative to the entire genome. Error bars represent the standard error of the mean, and the asterisks represent a p value < 0.001. b The number of putative gene sequences across the Glossina genus orthologous and paralogous to characterized MAG genes from G. m. morsitans. The genes are categorized by their functional classes as derived by orthology to characterized proteins from Drosophila and other insects. The functional classes include Novel—tsetse-specific genes; OBPs—odorant-binding proteins; peptidase—proteins with peptidase/-like functions; Unk.—proteins with orthologs in other insects that lack functional characterization