Fig. 5

Effects of the position of proximal dsgRNA on Cas9-TV editing. The distances were calculated based on the nucleotides that separate the sgRNA and dsgRNA target sites. Indel frequencies were measured by sequencing targeted amplicons in rice protoplasts. An untreated protoplast sample was used as a control. Data are from sets of three independent biological replicates (n = 3) and are shown as means ± s.e.m