Skip to main content
Fig. 3 | Genome Biology

Fig. 3

From: Modulating chromatin accessibility by transactivation and targeting proximal dsgRNAs enhances Cas9 editing efficiency in vivo

Fig. 3

Fusion of a synthetic transcription activator to Cas9 improves its editing efficiency. a Diagrams of the Cas9-TV construct. b Indel frequencies induced by Cas9 and Cas9-TV at the 20 target sites in rice protoplasts. An untreated protoplast sample was used as a control. Data are from sets of three independent biological replicates (n = 3) and are shown as means ± s.e.m. c Summary of the indel frequencies induced by Cas9 and Cas9-TV at the 20 target sites. d Summary of indel frequencies induced by Cas9 and Cas9-TV at target sites in open chromatin regions. e Summary of indel frequencies induced by Cas9 and Cas9-TV at target sites in closed chromatin regions. In c, d, and e, the distribution of indel frequencies is shown on a violin plot. The middle dashed line represents the mean. The outer dashed lines represent the interquartile ranges. P values were calculated from two-tailed Mann-Whitney tests. *P < 0.05, ***P < 0.001

Back to article page