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Fig. 1 | Genome Biology

Fig. 1

From: Modulating chromatin accessibility by transactivation and targeting proximal dsgRNAs enhances Cas9 editing efficiency in vivo

Fig. 1

Effect of chromatin accessibility on Cas9 genome editing efficiency in rice. a Summary of CRISPR/Cas9-mediated mutation numbers and chromatin accessibility at 70 target sites. Mutagenesis efficiency was measured on regenerated T0 rice plants by PCR/RE. The accessibility of each target site was obtained from the high-resolution maps of DNase I hypersensitive sites (DHS) of rice generated by Zhang et al. [19]. b Indel frequencies at 40 target sites in 20 rice genes examined in protoplasts. Two sites were targeted in each gene by independent sgRNAs. Indel frequencies were measured by sequencing targeted amplicons. Data are from sets of three independent biological replicates (n = 3) and are shown as means ± s.e.m. c Summary of indel frequencies and chromatin states at the 40 target sites in b. In a and c, the distribution of indel frequencies is shown on a violin plot. The middle dashed line represents the mean. The outer dashed lines represent the interquartile ranges. P values were calculated by two-tailed Mann-Whitney tests. **P < 0.01, ***P < 0.001

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