Fig. 4

Network analysis of long-read alignments and Hi-C intercontig links identifies hosts for assembled viral contigs. In order to identify putative hosts for viral contigs, PacBio read alignments (light blue edges) and Hi-C intercontig link alignments (dark blue edges) were counted between viral contigs (hexagons) and non-viral contigs (circles) in the long-read assembly (a) and the short-read assembly (b). Instances where both PacBio reads and Hi-C intercontig links supported a virus-host assignment are also labeled (red edges). The long-read assembly enabled the detection of more virus-host associations in addition to several cases where viral contigs may display cross-species infectivity. We identified several viral contigs that infect important species in the rumen, including those from the genus Sutterella, and several species that metabolize sulfur. In addition, we identified a candidate viral association with a novel genus of rumen microbes identified in this study