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Fig. 2 | Genome Biology

Fig. 2

From: Alternative splicing is required for stage differentiation in malaria parasites

Fig. 2

Genetic characterization of PbSR-MG. a Western blot and immunofluorescence assay of PbSR-MG-HA transgenic parasites. The western blot was probed with anti-HA, with the predicted mass of the chimeric HA protein at 89.0 kDa. Immunofluorescence assays were captured with confocal microscopy. The green channels shows localization of the HA-tagged PbSR-MG. The blue channels show DNA, which is stained with Hoechst 33342. The merge of these two follows, and the last column adds the brightfield differential interference contrast (DIC) image. b PCR screen for PbSR-MG KO vector integration into P. berghei ANKA, after monoclonal parasites were selected. c Quantification of alternatively spliced genes after ablation of PbSR-MG. After ablation, changes in alternative splicing (AS) can be quantified for each stage. The number of affected genes is low in asexual parasites, moderate in female gametocytes, and highest in males. d The total amount of alternative splicing (excluding intron retention) quantified for each stage independently. Total column heights represent total alternatively spliced genes detected in wild type (WT) or PbSR-MG KO (KO). The numbers of genes shared between these samples are indicated by a striped pattern

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