Fig. 1

System diagram of pan-genome construction subsystem in HUPAN. Seven processes include as follows: ① de novo assembly all reads into contigs, ② removing contigs similar to the human reference genome, ③ extracting unaligned sequences (including fully unaligned sequences and partially unaligned sequences), ④ merging unaligned sequences from multiple individuals, ⑤ removing redundant sequences, ⑥ removing potential contaminations, and ⑦ constructing pan-genome combining the human reference genome and novel sequences