Fig. 5

AIDA upregulation by TNFα is controlled by a regulatory element that includes one coronary artery disease-associated SNP. a Graphical representation of the transcriptomic, epigenomic, and 3D conformation data at the AIDA coronary artery disease (CAD)-associated locus. The CRISPR/Cas9 deletion is indicated in red and both qPCR assays are represented in green. We added gray vertical bars to highlight the TNFα-sensitive open chromatin peak (left) and the AIDA promoter (right). To improve visualization, we also increased the width of the arcs linking both elements (purple). b Relative AIDA expression in teloHAEC without or with TNFα treatment for 4 h with qPCR assay #1. Data was obtained from two independent experiments (circles in blue and cyan indicate the different biological replicates). For the non-deleted (Non-Del) and CRISPR/Cas9 heterozygote (Del) data points, we pooled data from three independent clones. Mean and standard deviation are plotted (black). For statistical analysis, we used linear regression correcting for batch effects and report two-tailed P values. c As for b, AIDA transcript quantification performed with qPCR assay #2