Fig. 6

Experimental validation of predicted enhancers. a, b The predicted enhancer loci were cloned into pMY155 reporter constructs (a) to examine their regulatory potential based on a luciferase reporter assay (b). Results for the first six predicted regions are presented, and results for the other 26 predicted regions are provided in Additional file 1: Figure S4. c Relative intensity of 26 predicted enhancer-like elements in states 5–7 and six active genic regions in states 1 and 2 in the reporter assay. Two control regions (ctrl) were randomly chosen from genomic regions without any histone modifications characterized in the present study. The order of the region tested is the same with the experimental results shown in Additional file 1: Figure S4 and the quantitative results listed in Additional file 2: Table S5. d Correlation between the relative intensity determined by the reporter assay and the ChIP-sequencing or DNase-sequencing read density in predicted enhancer-like regions of each epigenetic mark. Detailed data are listed in Additional file 2: Table S5. e Scatter plot presenting the correlation between the DHS read density and relative intensity in the reporter assay for the 32 predicted regions. Detailed data are listed in Additional file 2: Table S5