Fig. 2

Broad applications for CDetection. a (Left) Schematics showing the sequence variation within ABO genes and corresponding sgRNAs and tgRNAs. PAM sequences are colored in red, protospacers are colored in blue, SNPs are colored in pink, and base substitution in tgRNAs are colored in orange. (Right) CDetection combined with tgRNA achieve ABO blood genotyping detection with a single-nucleotide-resolution specificity. Error bars indicate standard errors of the mean (s.e.m.), n = 3. RFU, relative fluorescence units; tgRNA, tuned guide RNA. Two-tailed Student’s t test is used for significance analysis among each other in the group. b, c (Upper) Schematics showing the sequence variation within BRCA1 gene and targeting sgRNA and tgRNAs. (Lower) Maximum fluorescence signal showing the specificity of CDetection without RPA for human BRCA1 b 3232A>G and c 3537A>G mutation detection using sgRNA and tgRNA. Error bars indicate s.e.m., n = 3. Two-tailed Student’s t test is used for significance analysis among each other in the group. d Fluorescence timecourses showing the sensitivity and specificity of CDetection with RPA for human BRCA1 3232A>G mutation detection using tgRNA (3232-1). BRCA1 wild-type genomic DNAs or 3232A>G mutant genomic DNAs are extracted from cell lines. Error bars indicate s.e.m., n = 3. e Fluorescence timecourses showing the sensitivity and specificity of CDetection with RPA for human BRCA1 3232A>G mutation detection using tgRNA (3232-1). BRCA1 wild-type or 3232A>G dsDNAs are diluted in human plasma with a final concentration of 10⁻¹⁸ M. Error bars indicate s.e.m., n = 3. **P < 0.01; ****P < 0.0001; ns, no significance