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Table 1 Cell lines and culture conditions used in this study

From: CellSIUS provides sensitive and specific detection of rare cell populations from complex single-cell RNA-seq data

Cell line Gender Cell type Tissue of origin Obtained from Culture conditions
A549 M Alveolar basal epithelial (adherent) Lung adenocarcinoma ATCC
CCL-185
ATCC F12K (ATCC, P/N 30-2004) +10% FCS (AMIMED, P/N 2-01F36-I).
H1437 M Epithelial/glandular (adherent) Lung adenocarcinoma, derived from metastatic site: pleural effusion ATCC
CRL-5872
RPMI (Invitrogen, P/N A1049101) +10% FBS (ATCC, P/N SCRR-30-2020)
HCT116 M Epithelium-like (adherent) Colon carcinoma ATCC
CCL-247
ATCC McCoy's 5A (ATCC, P/N 30-2007) + 10% FCS (AMIMED, P/N 2-01F36-I)
HEK293 F Epithelial (adherent) Transformed cell line, derived from embryonic kidney ATCC, P/N CRL-1573 ATCC EMEM (ATCC, P/N 30-2003) +10% FCS (AMIMED, P/N 2-01F36-I)
IMR90 F Fibroblast (adherent) Fetal lung ATCC
CRL-186
ATCC EMEM (ATCC, P/N 30-2003) 10% FCS (AMIMED, P/N 2-01F36-I)
Jurkat M T cell (suspension) Childhood T acute lymphoblastic leukemia ATCC, P/N TIB-152 RPMI (Invitrogen, P/N 61870-044) + 10% FCS (AMIMED, P/N 2-01F36-I)
K562 F Undifferentiated, lymphoblast with granulocyte/erythrocyte/monocyte characteristics (suspension) Chronic myelogenous leukemia, BCR-ABL1 positive ATCC, P/N CRL-1573 RPMI (Invitrogen, P/N 61870-044) + 10% FCS (AMIMED, P/N 2-01F36-I).
Ramos M B cell (suspension) Burkitt’s lymphoma ATCC, P/N CRL-1596 Batch 3: RPMI (Invitrogen, P/N A1049101) +10% FBS (ATCC, P/N SCRR-30-2020)
Batch 4: RPMI (Invitrogen, P/N 61870-044) + 10% FCS (AMIMED, P/N 2-01F36-I)