Fig. 4
ZAM-derived piRNAs are produced from a pre-existing germline piRNA cluster in RevI-H2 ovaries. a–b Confocal images of ovarioles after GFP (green, left panels) and DNA (blue, middle panels) staining. Merged images of the GFP and DNA signals are displayed on the right. Ovarioles were from the progeny of a cross between RevI-H2 females and control males (ZAM maternal deposition, ZMD) in a and from a cross between RevI-H2 males and control females (No ZAM maternal deposition, NZMD) in b. In both crosses, the pGFP-ZAM line in which ZAM expression is driven in germline cells by a nanos-Gal4 driver was the control line. c Western blotting of proteins extracted from ovaries of progenies of crosses between wIR6 or RevI-H2 and the same control line as in a and b. The lines used for the crosses are indicated above. Proteins were from two biological replicates (1 and 2) prepared from 5 pairs of ovaries; α-tubulin was used as loading control. d Density profile of piRNAs mapping along the GFP-ZAM transgene sequence (allowing up to 3 mismatches). Sense and antisense reads are in black and grey, respectively. The profiles are for crosses between wIR6 (left, control) or RevI-H2 (right, ZMD) females and control males harboring the pGFP-ZAM transgene