Fig. 2

Deletion of some TE fragments in flamenco does not impair the global piRNA production from this piRNA cluster. a Pie chart showing the proportion of unique piRNAs that map to each of the 142 piRNA clusters in ovarian somatic sheath cells (no mismatch allowed, piRNA clusters defined as in [9]). b Density profile of unique piRNAs from the w^IR6 (left) and RevI-H2 (right) lines that map to the flamenco piRNA cluster. Sense and antisense reads are presented in black and grey, respectively. Almost no antisense reads map to the flamenco piRNA cluster. ZAM location in flamenco is displayed by a purple box. The flamenco deletion distal break-point in RevI-H2 [26] (Additional file 1: Figure S1B) is indicated by a red arrow and the sense of transcription by a black arrow. The count of piRNA reads per million (RPM) mapping the non-deleted region of flamenco, indicated below, does not differ between w^IR6 and RevI-H2