Fig. 6
Trans-silencing of transgenes. a Reciprocal crosses between silenced (S-TL) and active (A-TL) transgenic lines. Images were acquired from 14-day-old seedlings. b Area and mean “green” value were assessed by ImageJ. Student’s t tests were performed to assess significant differences between all quarters (SS, AA, AS, SA) of the diallel cross (FDRSSvsAA = 2.7 × 10−7, FDRSSvsSA = 1 × 10−8, FDRSSvsAS = 1 × 10−8, FDRAAvsSA = 0.87, FDRAAvsAS = 0.88, FDRSAvsAS = 0.47) (Additional file 1: Table S7). c Segregation in F2 seedlings. Chi-square tests were performed to test for deviation from Mendelian segregation (null-hypothesis 0.25/0.75 (sensitive/resistant), *0.05 > P ≥ 0.01, **0.01 > P ≥ 0.001, ***P < 0.001). Confidence interval indicates the range in which Mendelian segregation cannot be rejected. Bars with black triangles stem from pooled data of 4 individual F1 siblings (n = up to 4 × 52 seedlings), non-marked bars stem from mixed seeds of the 4 siblings (n = up to 52 seedlings). Gray bars: data not available (Additional file 1: Table S8, Table S9, Figure S4B)