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Fig. 6 | Genome Biology

Fig. 6

From: Single-cell transcriptomics unveils gene regulatory network plasticity

Fig. 6

Prediction of gene importance in DE data and directional changes in correlations. a Heatmap of normalized expression values of 911 genes found significantly upregulated in T2D β cells compared to healthy β cells (p < 0.05) sorted by decreasing Z-score (i.e., increasing p values) or decreasing centralities (betweenness, closeness, and degree). Biological importance of NEUROD1 and RCAN1 is highlighted by their high centrality but not by their DE Z-scores. In general, correlation between DE Z-scores and centrality is marginal, as shown by the erratic area plots of Z-scores sorted by centrality. b Pancreas regulatory network generated using all cells (both healthy and T2D), node size proportional to its degree. NEUROD1 and RCAN1 have high degree centrality (461 and 438 respectively). c Scatter plot and marginal distributions (MD1–4) of all pairwise gene correlations with |ρ| > 0.9 (329,046 couples). The strong bias in the marginal distributions (especially MD1, 2, 4) indicates an overall similarity of correlations between healthy and T2D pancreas. d An example of a highly conserved correlation (BMP5-PCSK1) against the strongest inversion (ZNF134-TFAMP1). e Overview of top 10 inversions of correlations. f Neither ZNF134 nor TFAMP1 display a significant change of expression (DE)

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